Literature Review: The Potential of Escherichia coli Bacteria As Insulin Producer
Keywords:
Diabetes melitus, Escherichia coli, InsulinAbstract
Diabetes mellitus is a chronic metabolic disease that requires the use of insulin as the main treatment, especially in people with type 1 diabetes and some cases of type 2 diabetes. Insulin plays an important role in regulating blood glucose levels by increasing glucose absorption by peripheral tissues and inhibiting glucose production by the liver. With the increasing need for insulin globally, recombinant insulin production technology using Escherichia coli bacteria has become an efficient, safe and economical alternative. This research is a literature review aimed at exploring the potential of E. coli as an expression system in the production of recombinant insulin. Based on the results of a review of several literatures, E. coli has several advantages such as fast growth, ease of genetic manipulation and the ability to produce insulin in large quantities with high quality. The application of genetic engineering technologies, plasmid systems, and protein expression has enabled the production of insulin that is purer and reduces immunological side effects compared to animal insulin. Thus, E. coli is a very potential and relevant platform in the development of recombinant insulin to meet global diabetes treatment needs.
References
Alyas, J., Rafiq, A., Amir, H., Khan, S. U., Sultana, T., Ali, A., Hameed, A., Ahmad, I., Kazmi, A., Sajid, T., & Ahmad, A. (2021). Human Insulin: History, Recent Advances, and Expression Systems for Mass Production. Biomedical Research and Therapy, 8(9), 4540–4561. https://doi.org/10.15419/bmrat.v8i9.692
Astutisari, I. D. A. E. C., Darmini, A. A. . Y., & Wulandari, I. A. P. (2022). Hubungan Pola Makan Dan Aktivitas Fisik Dengan Kadar Gula Darah Pada Pasien Diabetes Melitus Tipe 2 Di Puskesmas Manggis I. Jurnal Riset Kesehatan Nasional, 6(2), 79–87. https://doi.org/10.37294/jrkn.v6i2.350
Baeshen, N. A., Baeshen, M. N., Sheikh, A., Bora, R. S., Ahmed, M. M. M., Ramadhan, H. A. I., Salni, K. S., & Redwan, E. M. (2014). Cell factories for insulin production. Microbial Cell Factories, 13(141), 1–9. https://doi.org/10.1016/S0065-7743(08)60586-2
Datta, N. (2023). Indian Journal of Pharmaceutical and Biological Research (IJPBR) The Use of Escherichia coli for Recombinant Human Insulin Production. Indian J. Pharm. Biol. Res, 11(1), 1–3. https://doi.org/10.30750/ijpbr.11.1.02
Goeddel, D. V, Klepis, D. G., Bolivar, F., Heyneker, H. L., Yansura, D. G., Crea, R., Hirose, T., Kraszewski, A., Itakura, K., & Riggs, A. D. (1979). Expression in Escherichia coli of chemically synthesized genes for human insulin. Proceedings of the National Academy of Sciences of the United States of America, 76(1), 106–110. https://doi.org/10.1073/pnas.76.1.106
Govender, K., Naicker, T., Lin, J., Baijnath, S., Chuturgoon, A. A., Abdul, N. S., Docrat, T., Kruger, H. G., & Govender, T. (2020). A novel and more efficient biosynthesis approach for human insulin production in Escherichia coli (E. coli ). AMB Express, 10(43), 1–9. https://doi.org/10.1186/s13568-020-00969-w
Hardianto, D. (2020). Telaah Komprehensif Diabetes Melitus: Klasifikasi, Gejala, Diagnosis, Pencegahan, Dan Pengobatan. Jurnal Bioteknologi & Biosains Indonesia (JBBI), 7(2), 304–317. https://doi.org/10.29122/jbbi.v7i2.4209
International Diabetes Federation. (2017). In International Diabetes Federation (8th ed.). https://doi.org/10.24929/fp.v15i2.653
